牦牛AQP9基因CDS全长序列克隆及其生物信息学特征分析

doi:10.11843/j.issn.0366-6964.2015.07.008

Abstract

Abstract:

The aim of this study was to investigate the adaption mechanism of plateau animals to extreme environment of the Qinghai-Tibet plateau，and to provide some basic data for exploring the adaption mechanism of plateau animals.The coding region sequence of yak AQP9 was cloned by molecular cloning technique and analyzed by bioinformatics in this study.Some characteristics of the AQP9 gene and encoded protein sequences were predicted and analyzed in the following aspects as the general physical and chemical properties，hydrophobicity，transmembrane structure and secondary structure.The results showed that the CDS of yak AQP9 contains a complete ORF (885 bp) encoding 295 amino acids.The putative molecular weight and theory isoelectric point of AQP9 gene coding protein in yak were 31.89 ku and 6.21，respectively.The protein encoded by yak AQP9 contains 6 transmembrane domains，and it was a hydrophobic protein.The secondary structures are mainly composed of α-helix，extended strand，β-turn and random coil.Deduced amino acid sequences of AQP9 gene between yak and cattle，sheep and other species were high homology，and the phylogenetic distance consistent with their genetic relationship.This study will provide certain basic data for studying hypoxia adaptation of yak.

CLC Number:

S823.8⁺5.2

LIU Jian-feng，DING Yan-ping，WU Zhi-wei，WANG Jian-lin，SHAO Bao-ping. Cloning and Bioinformatics Analysis of the CDS of Yak AQP9 Gene[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(7): 1134-1140.

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Cloning and Bioinformatics Analysis of the CDS of Yak AQP9 Gene

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